A549 Lysate

Cat No.
ATGL0008

Size

QTY

List Price

100ug

USD 80


200ug

USD 110

500ug

USD 200

USD 0



Product Information

Product Type
Cell Lysate

Product Specification

Concentration
2mg/ml (determined by BCA assay)
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Storage
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Preparation before usage
Heat lysate to 95C for 5 minutes and rapidly cooling before use.
General Description
A549 cells are adenocarcinomic human alveolar basal epithelial cells. The A549 cell line was initiated from the removal and culturing of cancerous lung tissue in the explanted tumor from 58-year-old male, in 1972. A549 cells is able to synthesize lecithin and contain high level of unsaturated fatty acids, which are important to maintain the membrane phospholipids in cells. A549 cell line are widely used as an in vitro model for a type II pulmonary epithelial cell model for drug metabolism and as a transfection host.
Quality Control
Western Blot analyzed by both GAPDH and Beta tubulin antibody

Data

Western blot analysis (WB)

All lanes : A549 Whole Cell Lysate (40ug)

Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.