Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 27-202aa
- UniProt No.
- P01241
- NCBI Accession No.
- NP_072053.1
- Alternative names
- Somatotropin isoform 2, 20kDa Growth Hormone, GH, GH1, GHN, GH-N, Growth hormone, Growth hormone 1, hGH-N, Pituitary growth hormone, Somatotropin
Product Specification
- Molecular Weight
- 20 kDa (177aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range ≤1ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Human 20kDa Growth Hormone stimulates cell proliferation of the Nb2-11 Rat lymphoma cells. The ED50 range ≤1 ng/ml.
Related Publications
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Zhang H, et al. Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol-biotin chemiluminescent polymer. (Talanta. 2009)
Jung J, et al. Production and engineering of nanobody-based quenchbody sensors for detecting recombinant human growth hormone and its isoforms. (Drug Test Anal. 2023)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.