Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 158-316aa
- UniProt No.
- O35235
- NCBI Accession No.
- NP_035743
- Alternative names
- Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254
Product Specification
- Molecular Weight
- 17.9 kDa (160aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid In. 20mM Tris-HCl buffer (pH8.5) containing 0.1M NaCl
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Measured in a osteoclast differentiation assay using RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤2ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Mouse TRANCE/RANK L/TNFSF11 stimulates osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤2 ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.