Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 117-269aa
- UniProt No.
- P01584
- NCBI Accession No.
- NP_000567.1
- Alternative names
- Interleukin-1 beta, IL-1 beta, Catabolin, IL1F2
Product Specification
- Molecular Weight
- 17 kDa (154aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4)
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 12pg/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Human IL-1 beta /IL-1F2 stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range ≤ 12 pg/ml.
Related Publications
-
Choi DY, et al. Molecularly imprinted polymer-based electrochemical impedimetric sensors on screen-printed carbon electrodes for the detection of trace cytokine IL-1β. (Biosens Bioelectron. 2022)
Park RW, et al. Mobile Point-of-Care Device Using Molecularly Imprinted Polymer-Based Chemosensors Targeting Interleukin-1β Biomarker. (Biosensors. 2023)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.