Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 207-327aa
- UniProt No.
- P15692
- NCBI Accession No.
- NP_001020541
- Alternative names
- Vascular endothelial growth factor A isoform f, Vascular permeability factor, VPF, VEGF
Product Specification
- Molecular Weight
- 16.3 kDa (142aa) confirmed by MALDI-TOF
- Concentration
- 0.5mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 range ≤12ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Human VEGF121 stimulates cell proliferation of the HUVEC human umbilical vein endothelial cells. The ED50 range ≤12 ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.