Product Information
- Product Type
- Recombinant Protein
- Expression System
- Baculovirus
- Domain
- 22-401aa
- UniProt No.
- O00300
- NCBI Accession No.
- NP_002537.3
- Alternative names
- Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin, OCIF, OPG, TR1
Product Specification
- Molecular Weight
- 44.7 kDa (389aa)
(On SDS-PAGE under denatured condition, apparent Molecular weight of glycosylated rhOPG protein will appear at approximately 55 kDa)
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Measured by its ability to inhibit cytotoxicity using Jurkat human acute T cell leukemia cells in the presence of 2ng/ml of human TRAIL (CAT# ATGP3588). The ED50 range ≤ 8 ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
Biological Activity
Human Human OPG/TNFRSF11B inhibits Human TRAIL (CAT# ATGP3588) cytotoxicity in the Jurkat human acute T cell leukemia cells. The ED50 range ≤ 8 ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.