Recombinant e.coli G6PD protein

Cat No.
GPD0905

Size

QTY

List Price

20ug

USD 55


100ug

USD 170

500ug

USD 505

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
E.coli
Domain
1-491aa
UniProt No.
P0AC53
NCBI Accession No.
NP_416366
Alternative names
Glucose-6-phosphate 1-dehydrogenase, Glucose-6-phosphate 1-dehydrogenase, zwf, Glucose-6-phosphate 1-dehydrogenase G6PD, G6PD1, G6pdx, Glucose 6 phosphate 1 dehydrogenase, MET19, POS10, Zwf1p.

Product Specification

Molecular Weight
55.7 kDa (491aa) confirmed by MALDI-TOF
Concentration
1mg/ml (determined by Bradford assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 50unit/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
Tag
Non-Tagged
Applications
Enzyme Activity,SDS-PAGE
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.