Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-222aa
- UniProt No.
- P08263
- NCBI Accession No.
- NP_665683
- Alternative names
- Glutathione S transferase A1, Glutathione S-transferase alpha 1, GST epsilon, GST2, GSTA1, GSTA1-1, GTH1, HA subunit 1
Product Specification
- Molecular Weight
- 25.6 kDa (222aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity is > 35,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.