Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-352aa
- UniProt No.
- Q8IXJ6
- NCBI Accession No.
- NP_085096
- Alternative names
- NAD-dependent protein deacetylase sirtuin-2, NAD-dependent protein defatty-acylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIR2L2, Sirtuin, Silent mating type information regulation 2 homolog 2 (S. cerevisiae) cerevisiae)
Product Specification
- Molecular Weight
- 41.6 kDa (372aa) confirmed by MALDI-TOF
- Concentration
- 0.25mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 2mM DTT, 200mM NaCl, 0.5mM EDTA
- Purity
- > 90% by SDS-PAGE
- Tag
- His-Tag
- Applications
- SDS-PAGE
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Related Publications
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Kawaguchi M, et al. A Fluorescent Probe for Imaging Sirtuin Activity in Living Cells, Based on One-Step Cleavage of the Dabcyl Quencher. (Chembiochem. 2016)
Kawaguchi M, et al. Development of Peptide-Based Sirtuin Defatty-Acylase Inhibitors Identified by the Fluorescence Probe, SFP3, That Can Efficiently Measure Defatty-Acylase Activity of Sirtuin. (J Med Chem. 2019 )
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.