Recombinant human Isocitrate Dehydrogenase 1/IDH1 protein

Cat No.
ATGP1284

Size

QTY

List Price

20ug

USD 80


100ug

USD 240

500ug

USD 720

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
E.coli
Domain
1-414aa
UniProt No.
O75874
NCBI Accession No.
NP_005887.2
Alternative names
Isocitrate dehydrogenase [NADP] cytoplasmic, IDCD, IDH, IDP, IDPC, PICD

Product Specification

Molecular Weight
48.8 kDa (434aa) confirmed by MALDI-TOF
Concentration
1mg/ml (determined by Bradford assay)
Formulation
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT,0.1mM PMSF
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 18,000pmol/min/ug. One unit will oxidize 1.0pmole of DL-Isocitrate to D alpha-Ketoglutarate per minute in the presence of beta-NADP at pH7.4 at 37C.
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.