Product Information
- Product Type
- Recombinant Protein
- Expression System
- Baculovirus
- Domain
- 1-515aa
- UniProt No.
- P11413
- NCBI Accession No.
- NP_001035810.1
- Alternative names
- glucose-6-phosphate 1-dehydrogenase, G6PD1, G6PDH
Product Specification
- Molecular Weight
- 61.4 kDa (535aa)
- Concentration
- 0.5mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1mM PMSF, 2mM EDTA, 2mM DTT, 200mM NaCl
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 45unit/mg, obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.