Recombinant human IL-12 protein

Cat No.
ATGP2843

Size

QTY

List Price

10ug

USD 80


50ug

USD 240

250ug

USD 960

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
Baculovirus
Domain
23-328aa(p40)/23-219aa(p35)
UniProt No.
P29460(p40)/P29459(p35)
NCBI Accession No.
NP_002178(p40)AAK84425(p35)
Alternative names
Interleukin 12 (subunit beta/alpha), NKSF1

Product Specification

Molecular Weight
34.6 kDa (p40, 306aa)
23.3 kDa (p35, 203aa)
The heterodimeric IL12 has a predicted Molecular weight of 57.9 kDa (509aa). Molecular weight on SDS-PAGE will appear higher.
Concentration
0.5mg/ml (determined by absorbance at 280nm)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity
> 90% by SDS-PAGE
Bioactivity
The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤1ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Biological Activity

Human IL12 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ≤1 ng/ml.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.