Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 109-266aa
- UniProt No.
- Q8BVZ5
- NCBI Accession No.
- NP_001158196.1
- Alternative names
- Interleukin-33, IL-33, Il1f11, NF-HEV, 230117N10Rik
Product Specification
- Molecular Weight
- 18.1 kDa (181aa) confirmed by MALDI-TOF
- Concentration
- 0.5mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.1ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Mouse IL-33 stimulates cell proliferation D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.1ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.