Recombinant human TNF-alpha protein

Cat No.
ATGP3152

Size

QTY

List Price

10ug

USD 80


50ug

USD 240

250ug

USD 960

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
Baculovirus
Domain
77-233aa
UniProt No.
P01375
NCBI Accession No.
NP_000585
Alternative names
Tumor necrosis factor, DIF, TNF-alpha, TNFA, TNFSF2

Product Specification

Molecular Weight
18.1 kDa (163aa)
Concentration
1mg/ml (determined by absorbance at 280nm)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is less or equal to 0.2ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. ..

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.