Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-496aa
- UniProt No.
- P24298
- NCBI Accession No.
- NP_005300.1
- Alternative names
- alanine aminotransferase 1, AAT1, ALT1, GPT1
Product Specification
- Molecular Weight
- 56.8 kDa (516aa) confirmed by MALDI-TOF
- Concentration
- 0.5mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 100unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.