Product Information
- Product Type
- Recombinant Protein
- Expression System
- Baculovirus
- Domain
- 1-230aa
- UniProt No.
- Q9JKB1
- NCBI Accession No.
- NP_057932
- Alternative names
- Ubiquitin carboxyl-terminal hydrolase isozyme L3, Ubiquitin carboxyl-terminal esterase L3, Ubiquitin thioesterase L3, UCH-L3
Product Specification
- Molecular Weight
- 27.2 kDa (238aa) 28-40kDa (SDS-PAGE under reducing conditions)
- Concentration
- 0.5mg/ml (determined by absorbance at 280nm)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity is > 9,000pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0pmole of ubiquitin-AMC per minute at pH 7.5, at 37C.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.