Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 114-281aa
- UniProt No.
- P50591
- NCBI Accession No.
- NP_003801
- Alternative names
- Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, Apo-2L, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD253, APO2L, TL2, TANCR
Product Specification
- Molecular Weight
- 19.6 kDa (169aa)
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 300mM NaCl, 0.1mM DTT, 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured in a cytotoxicity assay using Jurkat human acute T cell leukemia cells. The ED50 range ≤1ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Human TRAIL/TNFSF10 stimulates cell cytotoxicity in the Jurkat human acute T cell leukemia cells. The ED50 range ≤1 ng/ml.
Related Publications
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Park D, et al. Morusin Induces TRAIL Sensitization by Regulating EGFR and DR5 in Human Glioblastoma Cells. (Journal of Natural Products. 2016)
Kim J, et al. Decursin enhances TRAIL-induced apoptosis through oxidative stress mediated- endoplasmic reticulum stress signalling in non-small cell lung cancers. (Br J Pharmacol. 2016)
Kim EO, et al. CCR4‑NOT transcription complex subunit 2 regulates TRAIL sensitivity in non‑small‑cell lung cancer cells via the STAT3 pathway. (Int J Mol Med. 2020)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.