Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 25-505aa
- UniProt No.
- P27773
- NCBI Accession No.
- NP_031978
- Alternative names
- Protein disulfide isomerase associated 3, 58 kDa glucose-regulated protein, GRP58, 58 kDa microsomal protein, p58, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, Erp, PDI, PDI-Q2, Plca
Product Specification
- Molecular Weight
- 56.8 kDa (505aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, and 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is >10 A650/cm/min/mg,obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reduction of insulin.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.