Recombinant mouse TNF-alpha protein

Cat No.
ATGP3651

Size

QTY

List Price

10ug

USD 160


20ug

USD 280

50ug

USD 460

100ug

USD 770

250ug

USD 1,340

500ug

USD 2,300

1mg

USD 3,840

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
E.coli
Domain
80-235aa
UniProt No.
P06804
NCBI Accession No.
NP_038721
Alternative names
Tumor necrosis factor, DIF, TNF-alpha, TNFalpha, Tnfsf1a, TNFSF2, Cachectin, ATGP3738
Additional Information
ATGP3738 has been replaced with a catalog number A

Product Specification

Molecular Weight
17.4 kDa (157aa) confirmed by MALDI-TOF
Concentration
1mg/ml (determined by Bradford assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤0.06ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Biological Activity

Mouse TNF-alpha stimulates cell cytotoxicity in the L929 mouse fibrosarcoma cell. The ED50 range ≤ 0.06ng/ml.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.