Recombinant mouse IL12 protein

Cat No.
ATGP3739

Size

QTY

List Price

10ug

USD 105


50ug

USD 320

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
Baculovirus
Domain
23-335aa(p40)/23-215aa(p35)
UniProt No.
P43432(p40)/P43431(p35)
NCBI Accession No.
NP_001290173(p40)/NP_032377(p35)
Alternative names
Interleukin 12 (subunit beta/alpha), IL12b/IL12a, Il-12b/Il-12a, IL-12p40/Il-12p35, Il12p40/Il12p35, p40/p35

Product Specification

Molecular Weight
35.7 kDa (p40, 313aa)
22.5 kDa (p35, 199aa)
The heterodimeric Il12 has a predicted Molecular weight of 58.3 kDa (512aa).
Molecular weight on SDS-PAGE will appear higher
Concentration
0.5mg/ml (determined by Bradford assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity
> 95% by SDS-PAGE
Bioactivity
The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 for this effect is less or equal to 10ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Applications
SDS-PAGE,Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE visualized by coomassie blue stain.
Lane 1: reducing condition
Lane 2: non-reducing condition ..

Biological Activity

Mouse IL12 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range is less or equal to 10 ng/ml.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.