Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-917aa
- UniProt No.
- P52789
- NCBI Accession No.
- NP_000180
- Alternative names
- HKII, HXK2
Product Specification
- Molecular Weight
- 104.5 kDa (937aa)
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl , 0.1mM PMSF
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity is > 40,000pmol/min/ug. One unit will convert 1pmole of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37C.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Related Publications
-
Wuest M, et al. Radiopharmacological evaluation of 6-deoxy-6-[18F]fluoro-D-fructose as a radiotracer for PET imaging of GLUT5 in breast cancer. (Nucl Med Biol. 2011)
Tsumura Y, et al. TMG-123, a novel glucokinase activator, exerts durable effects on hyperglycemia without increasing triglyceride in diabetic animal models. (PLoS One. 2017)
Ciscato F, et al. Hexokinase 2 displacement from mitochondria-associated membranes prompts Ca 2+ -dependent death of cancer cells. (EMBO Rep. 2020)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.