Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 115-270aa
- UniProt No.
- P16598
- NCBI Accession No.
- NP_058715
- Alternative names
- Interleukin-1 alpha precursor, Interleukin-1 alpha, IL1F1, IL-1A, IL-1 alpha
Product Specification
- Molecular Weight
- 20.2 kDa (179aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by absorbance at 280nm)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range ≤ 20 pg/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Rat IL-1 alpha in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range ≤ 20 pg/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.