Product Information
- Product Type
- Recombinant Protein
- Expression System
- Baculovirus
- Domain
- 20-478aa
- UniProt No.
- P04004
- NCBI Accession No.
- NP_000629
- Alternative names
- VTN, VN, S-protein, Serum-spreading factor, V75, Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B, Somatomedin B, Complement S-protein
Product Specification
- Molecular Weight
- 53.3 kDa (468aa)
- Concentration
- 0.25mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Measured by the ability of the immobilized protein to support the adhesion of B16-F10 mouse melanoma cells. When cells are added to VTN coated plates 5ug/ml. This effect is more to 50%.
- Endotoxin
- < 0.1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.