Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 78-234aa
- UniProt No.
- Q06599
- NCBI Accession No.
- NP_776391
- Alternative names
- Tumor necrosis factor, TNF-a, TNF, TNFa
Product Specification
- Molecular Weight
- 17.5 kDa (158aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 15ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Bovine TNF-alpha stimulates cell cytotoxicity in the L929 mouse fibrosarcoma cells. The ED50 range ≤ 15ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.