Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 113-271aa
- UniProt No.
- P01583
- NCBI Accession No.
- NP_000566
- Alternative names
- Interleukin 1 alpha, preinterleukin 1 alpha, Hematopoietin-1, Pro-interleukin-1-alpha, IL1F1, IL-1A, IL1-ALPHA, IL1
Product Specification
- Molecular Weight
- 22.4 kDa (197aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by absorbance at 280nm)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 for this effect is less or equal to 0.04ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Human IL-1 alpha /IL-1F1 stimulates cell proliferation of the D10.G4.1 mouse helper T cell. The ED50 range is less or equal to 0.04 ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.