NKMAX

Product Information

Product Type

Recombinant Protein

Expression System

E.coli

Domain

283-396aa

UniProt No.

P12643

NCBI Accession No.

NP_001191

Alternative names

Bone morphogenetic protein 2, BMP-2A

Product Specification

Molecular Weight

13 kDa (115aa)

Concentration

1mg/ml (determined by Bradford assay)

Formulation

Liquid in. 10mM Sodium acetate (pH 3.5)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is ≤ 100 ng/ml.

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

Magnify

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
Lane1 : reducing conditions
Lane2 : non-reducing conditions

Biological Activity

Magnify

Human BMP-2 stimulates alkaline phosphatase in the ATDC5 mouse chondrogenic cells. The ED50 range ≤ 100 ng/ml.

Related Publications

Tanaka M, et al. Physico-Chemical, In Vitro, and In Vivo Evaluation of a 3D Unidirectional Porous Hydroxyapatite Scaffold for Bone Regeneration. (Materials (Basel). 2017)
Tanaka M, et al. In Vitro and In Vivo Evaluation of a Three-Dimensional Porous Multi-Walled Carbon Nanotube Scaffold for Bone Regeneration. (Nanomaterials (Basel). 2017)
Qi S, et al. Cellular Internalization-Induced Aggregation of Porous Silicon Nanoparticles for Ultrasound Imaging and Protein-Mediated Protection of Stem Cells. (Small. 2019)