MERS-CoV Spike RBD antibody

Cat No.
ATGA0595

Size

QTY

List Price

50ul

USD 170


100ul

USD 290

USD 0



Product Information

Product Type
Monoclonal antibody
Clone Number
AT2F7
UniProt No.
K0BRG7
NCBI Accession No.
AFS88936
Alternative names
Middle East respiratory syndrome coronavirus, Human betacoronavirus 2c EMC/2012, MERS-CoV, MERS, MERS-CoV RBD, MERS RBD, receptor binding domain, RBD, Spike RBD protein

Product Specification

Host
Mouse
Reacts With
MERS-CoV
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant MERS-CoV Spike RBD (358-606aa) purified from Baculovirus
Isotype
IgG2b kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB
Usage
The antibody has been tested by ELISA analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MERS Spike RBD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant MERS Spike protein (ATGP3979)
Lane 2.: Recombinant MERS Spike S1 protein (ATGP3980)
Lane 3.: Recombinant MERS Spike S2 protein (ATGP3981)
Lane 4.: Recombinant MERS Spike RBD protein (ATGP3982)

Additional Information

ELISA: MERS Spike RBD Antibody (1ug/ml) specifically recognizes MERS Spike, Spike S1 and Spike RBD recombinant protein, but not interacted MERS Spike S2 recombinant protein in ELISA.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.