Product Information
- Product Type
- Recombinant Protein
- Expression System
- HEK293
- Domain
- 28-232aa
- UniProt No.
- Q13253
- NCBI Accession No.
- NP_005441.1
- Alternative names
- SYM1, SYNS1, SYNS1A, NOG, symphalangism 1, synostoses syndrome 1
Product Specification
- Molecular Weight
- 23.8kDa (211aa)
- Concentration
- 0.25mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 95% by SDS - PAGE
- Bioactivity
- Measured by ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells in the presence of 50ng/ml of human BMP-4. The ED50 range ≤ 0.5 ug/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
Biological Activity
Human Nogging inhibit alkaline phosphatase production in the ATDC5 mouse chondrogenic cells in the presence of 50ng/ml of human BMP-4. The ED50 range ≤ 0.5 ug/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.