Product Information
- Product Type
- Recombinant Protein
- Expression System
- HEK293
- Domain
- 29-766aa
- UniProt No.
- P27487
- NCBI Accession No.
- NP_001926.2
- Alternative names
- Dipeptidyl peptidase 4, dipeptidyl peptidase 4 isoform 1, ADABP, ADCP2, CD26, DPPIV, TP103, Adenosine deaminase complexing protein 2, Dipeptidyl peptidase IV, DPP4, T-cell activation antigen CD26
Product Specification
- Molecular Weight
- 112.1kDa (977aa)
- Concentration
- 0.25mg/ml (determined by Absorbance at 280nm)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- 1.Measured by its binding ability in a functional ELISA with MERS-CoV Spike S1 Subunit (CAT# ATGP3980). The ED50 range ≤250 ng/ml.
2.Measured by its binding ability in a functional ELISA with MERS-CoV Spike RBD (CAT# ATGP4111). The ED50 range ≤200 ng/ml.
3.Measured by its binding ability in a functional ELISA with MERS-CoV Spike (CAT# ATGP4112). The ED50 range ≤120 ng/ml.
4.Specific activity is > 15,000 pmol/min/ug in which one unit defined as the amount of enzyme that hydrolyze 1pmole of H-Gly-Pro-AMC.HBr to H-Gly-Pro and AMC per minute at pH 8.0 at 37℃.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- hIgG-Tag
- Applications
- SDS-PAGE, Enzyme Activity, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
Biological Activity
MERS-CoV Spike S1 Subunit (CAT# ATGP3980) is coated at 2 ug/ml (100 ul/well) can bind IgK-DPP4. The ED50 range ≤250 ng/ml.
MERS-CoV Spike RBD (CAT# ATGP4111) is coated at 2 ug/ml (100 ul/well) can bind IgK-DPP4. The ED50 range ≤200 ng/ml.
MERS-CoV Spike (CAT# ATGP4112) is coated at 2 ug/ml (100 ul/well) can bind IgK-DPP4. The ED50 range ≤120 ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.