Product Information
- Product Type
- Recombinant Proteins
- Expression System
- E.coli
- Domain
- 140-317aa
- UniProt No.
- O14788
- NCBI Accession No.
- NP_003692.1
- Alternative names
- Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254
Product Specification
- Molecular Weight
- 22.3 kDa (199aa) confirmed by MALDI-TOF
- Concentration
- 0.5mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
- Purity
- > 85% by SDS – PAGE
- Bioactivity
- Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤ 25 ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Human TRANCE stimulates osteoclast differentiation in the RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤ 25 ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.