Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT7B4
- UniProt No.
- Q9BZ95
- NCBI Accession No.
- NP_075447
- Alternative names
- Transcription elongation factor B polypeptide 3 binding protein 1, DKFZp667H044, FLJ20353, Histone lysine N methyltransferase NSD3, MGC126766, MGC142029, NSD 3, Nuclear set domain containing 3, Nuclear SET domain containing protein 3, pp14328, Whistle, WHSC1 like protein 1, WHSC1L1 protein, Wolf Hirschhorn syndrome candidate 1 like 1,
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human NSD3 (383-660aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA,WB
- Usage
- The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Western blot analysis (WB)
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSD3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: MCF7 cell lysate
Lane 4.: NIH3T3 cell lysate
Lane 5.: U87MG cell lysate
Lane 1.: 293T cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: MCF7 cell lysate
Lane 4.: NIH3T3 cell lysate
Lane 5.: U87MG cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.