NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

2E4

UniProt No.

P10809

NCBI Accession No.

NP_002147

Alternative names

Heat shock 60kDa protein 1, CPN60, GroEL, HSP65, SPG13, HuCHA60, Heat shock 60kDa protein 1, HSP60, Heat shock 60kDa protein 1 60 kDa chaperonin, GroEL, E, coli, homolog of, 60 kDa heat shock protein mitochondrial, 60kDa, cb863, Chaperonin, Chaperonin 60, Chaperonin, 60-KD, CPN 60, fa04a05, fb22d10, fi27b05, GroEL Homolog, Heat shock 60kD protein 1 (chaperonin), Heat shock 60kD protein 1 chaperonin, heat shock 60kDa protein 1 (chaperonin), Heat shock protein 1 (chaperonin), Heat Shock Protein 60, Heat shock protein 65, HLD4, Hsp 60, HSP 65, HSPD 1, HSPD1, HuCHA60, id:ibd2197, Spastic paraplegia 13 Mitochondrial matrix protein P1, P60 lymphocyte protein, sb:cb144, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (autosomal dominant), SPG 13, wu:fa04a05, wu:fb22d10, wu:fi04a12, wu:fi27b05.

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human Hsp60 (1-573aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, ICC/IF, IHC

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of Hsp60 in HeLa cells. The cell was stained with AHS0815 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp60 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: A549 cell lysate

Immunohistochemistry (IHC)

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Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp60 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)