NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT4G6

UniProt No.

P60709

NCBI Accession No.

NP_001092

Alternative names

Actin, cytoplasmic 1, Beta-actin

Product Specification

Host

Mouse

Reacts With

Human

Concentration

0.2mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human ACTB (1-375aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: Jurkat cell lysate
Lane 3.: PC3 cell lysate
Lane 4.: HepG2 cell lysate
Lane 5.: MCF7 cell lysate
Lane 6.: U87-MG cell lysate
Lane 7.: NIH-3T3 cell lysate

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The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Kidney tissue lysate
Lane 2.: Liver tissue lysate
Lane 3.: Spleen tissue lysate
Lane 4.: Bone marrow tissue lysate
Lane 5.: Stomach tissue lysate

Magnify

The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: ACTB antibody 1:1000
Lane 2.: ACTB antibody 1:3000
Lane 3.: ACTB antibody 1:5000
Lane 4.: ACTB antibody 1:10000

Related Publications

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Yang YJ et al., Korean Mistletoe (Viscum album var. coloratum) Ethanol Extracts Enhance Intestinal Barrier Function and Alleviate Inflammation. (Antioxidants (Basel) 2025)
Kim MJ et al., Potential Chondroprotective Effect of Artemisia annua L. Water Extract on SW1353 Cell. (Int. J. Mol. Sci. 2025)
Han JY et al., Cytoplasmic HMGB1 promotes the activation of JAK2-STAT3 signaling and PD-L1 expression in breast cancer. (Mol Med. 2025)
Kim MJ et al., Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibition. (Sci Rep. 2025)