NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT19F2

UniProt No.

Q9NR28

NCBI Accession No.

NP_063940

Alternative names

Diablo isoform 1, SMAC3, DIABLO-S, FLJ10537, FLJ25049, Diablo isoform 1, SMAC/Diablo, Diablo isoform 1 0610041G12Rik, DBOH, Diablo homolog, DIABLO S, Diablo homolog (Drosophila), Mitochondrial Smac protein, Diablo homolog mitochondrial, SMAC 3, Smac protein, Direct IAP binding protein with low pI, Second mitochondria derived activator of caspase

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human SMAC/Diablo (56-239aa) purified from E. coli

Isotype

IgG2a kappa

Purification

By protein-A affinity chromatography

Applications

ELISA,WB,ICC/IF,FACS

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of SMAC in HeLa cells. The cell was stained with ATGA0266 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The cell lysate of Jurkat (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SMAC antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SMAC antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: A431 cell lysate
Lane 3.: Hep3B cell lysate
Lane 4.: MCF7 cell lysate

Flow cytometry (FACS)

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Flow cytometry analysis of SMAC in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).