NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT3F9

UniProt No.

P04075

NCBI Accession No.

NP_908930

Alternative names

Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDA

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human ALDOA (1-364aa) purified from E. coli

Isotype

IgG2a kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF, FACS

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of ALDOA in A549 cells. The cell was stained with ATGA0341 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The Recombinant protein and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human ALDOA
Lane 2.: HeLa cell lysate
Lane 3.: HepG2 cell lysate

Flow cytometry (FACS)

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Flow cytometry analysis of ALDOA in A549 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).