NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT2E11

UniProt No.

P09972

NCBI Accession No.

NP_005156

Alternative names

Aldolase C fructose-bisphosphate., Aldolase C, fructose-bisphosphate., ALDC

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human ALDOC (1-364aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA,WB,ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of ALDOC in U87MG cells. The cell was stained with ATGA0397 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

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ICC/IF analysis of ALDOC in Jurkat cells. The cell was stained with ATGA0397 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOC antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : PC3 cell lysate
Lane 2. : U87MG cell lysate
Lane 3. : WiDr cell lysate
Lane 4. : Jurkat cell lysate
Lane 5. : Mouse brain tissue lysate