NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT2C10

UniProt No.

P05091

NCBI Accession No.

NP_000681

Alternative names

Aldehyde dehydrogenase 2 family member, Aldehyde dehydrogenase mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDM

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human ALDH2 (18-517aa) purified from E. coli

Isotype

IgG2a kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of ALDH2 in A549 cells line, stained with monoclonal anti-human ALDH2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

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The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HepG2 cell lysate
Lane 2. : Mouse liver tissue lysate
Lane 3. : Mouse lung tissue lysate

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH2 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : 293T cell lysate
Lane 2. : ALDH2 transfected 293T cell lysate