Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT2C10
- UniProt No.
- P05091
- NCBI Accession No.
- NP_000681
- Alternative names
- Aldehyde dehydrogenase 2 family member, Aldehyde dehydrogenase mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDM
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human ALDH2 (18-517aa) purified from E. coli
- Isotype
- IgG2a kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of ALDH2 in A549 cells line, stained with monoclonal anti-human ALDH2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western blot analysis (WB)
The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HepG2 cell lysate
Lane 2. : Mouse liver tissue lysate
Lane 3. : Mouse lung tissue lysate
Lane 1. : HepG2 cell lysate
Lane 2. : Mouse liver tissue lysate
Lane 3. : Mouse lung tissue lysate
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH2 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : 293T cell lysate
Lane 2. : ALDH2 transfected 293T cell lysate
Lane 1. : 293T cell lysate
Lane 2. : ALDH2 transfected 293T cell lysate
Related Publications
-
Kim MJ et al., Cinnamomum verum extract as a potential therapeutic for atopic dermatitis through reducing lipid peroxidation and oxidative stress. (Front Pharmacol. 2026)
Kim MJ et al., Ulmus pumila Linné (Ulmi) Extract Attenuates Inflammatory Responses in Atopic Dermatitis by Modulating Lipid Peroxidation and Oxidative Stress. (Antioxidants. 2026)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.