Product Information
- Product Type
- Cell Lysate
Product Specification
- Concentration
- 2mg/ml (determined by BCA assay)
- Formulation
- Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
- Storage
- Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
- Preparation before usage
- Heat lysate to 95C for 5 minutes and rapidly cooling before use.
- General Description
- SW480 cells was established from a primary adenocarcinoma of the colon in a 50 year old male. SW480 cells are epithelial like in morphology and used as an in vitro model for colorectal cancer to study tumor markers, CEA production, biochemistry of tumorigenicity, pharmacodynamics, hormone sensibility and as a positive control for the nuclear protein Beta catenin.
- Quality Control
- Western Blot analyzed by both GAPDH and Beta tubulin antibody
Data
Western blot analysis (WB)
All lanes : SW480 Whole Cell Lysate (40ug)
Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)
Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.