Human C/EBP-Beta antibody

Cat No.
ABP0609

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
47A1
UniProt No.
P17676
NCBI Accession No.
NP_005185
Alternative names
CEBPB(CCAAT/enhancer binding protein, beta), LAP, CRP2, TCF5, IL6DBP, NF-IL6, MGC32080,C/EBP-beta, CCAAT/enhancer binding protein (C/EBP) beta, NFIL6, liver-enriched transcriptional activator protein, nuclear factor of interleukin 6, interleukin 6-dependent DNA-binding protein,

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human C/EBP-beta (1-271 aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of C/EBP-beta in HaLa cells. The cell was stained with ABP0609 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C/EBP-beta antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.