Human NPM1 antibody

Cat No.
ANP0626

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
5E3.
UniProt No.
P06748
NCBI Accession No.
NP_002511
Alternative names
nucleophosmin (nucleolar phosphoprotein B23,numatrin),B23, NPM, MGC104254, nucleolar phosphoprotein B23, numatrin, nucleophosmin/nucleoplasmin family, member 1

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human NPM (81-294aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, ICC/IF, IHC, FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of NPM in HeLa cells. The cell was stained with ANP0626 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The HeLa and HepG2 cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow cytometry (FACS)

Flow cytometry analysis of NPM in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

IHC Comment And Pic

This image shows paraffin-embedded human palatine tonsil tissue sample stained with anti-NPM antibody (5E3) at 1:100 dilution.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.