Human SNAP25 antibody

Cat No.
ASN0720

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
4E11
UniProt No.
P60880
NCBI Accession No.
NP_570824
Alternative names
Synaptosomal-associated protein 25 isoform SNAP25B, SNAP25, SNAP, SNAP25, Synaptosomal-associated protein, His- tag, SNAP-25, Synaptosomal-associated 25 kDa protein, Super protein, SuP, Synaptosomal-associated protein 25, Synaptosomal-associated protein 25 isoform SNAP25B bA416N4.2, Bdr, dJ1068F16.2, FLJ23079, HGNC:11132, MGC105414, MGC139754, Resistance to inhibitors of cholinesterase 4 homolog, RIC 4, RIC4, SEC9, SNAP 25, SNAP-25B, SNP 25, sp, Synaptosomal associated 25 kDa protein, Synaptosomal associated protein, Synaptosomal Associated Protein 25, Synaptosomal associated protein 25kDa

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human SNAP25 (1-206aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

ICC/IF analysis of SNAP25 in U87MG cells. The cell was stained with ASN0720 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The tissue lysate (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse brain tissue lysate
The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: SNAP25 Transfected 293T cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.