Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- 1D1
- UniProt No.
- Q99828
- NCBI Accession No.
- NP_006375
- Alternative names
- Calcium and integrin binding 1, CIB, KIP (Kinase interacting protein), SIP2-28(SNK interacting protein2-28), CALMYRIN, CIB1, Calcium and integrin binding 1 Calcium and integrin binding 1 (calmyrin), Calcium and integrin binding protein 1, CIB, DNA PKcs interacting protein, Human Snk interacting protein 2 to 28 mRNA complete cds, Kinase interacting protein, PRKDCIP, SIP2 28, SNK interacting protein 2 to 28
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human CIB1 (1-191aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA,WB,ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of CIB1 in HeLa cells. The cell was stained with ACI0807 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysate of HT29 (40ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CIB1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CIB1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: CIB1 Transfected 293T cell lysate
Lane 1.: 293T cell lysate
Lane 2.: CIB1 Transfected 293T cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.