Human Podoplanin antibody

Cat No.
APD0833

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
5E2
UniProt No.
Q86YL7
NCBI Accession No.
NP_006465
Alternative names
Lung type-I cell membrane-associated glycoprotein isoform a, T1A, GP36, GP40, Gp38, OTS8, T1A-2, HT1A-1, PA2.26, Lung type-I cell membrane-associated glycoprotein isoform a, Podoplanin, PDPN, Lung type-I cell membrane-associated glycoprotein isoform a AGGRuS, Glycoprotein 36 KD, Glycoprotein 36, gp 36, GP 38, GP 40, GP40, HT1A 1, HT1A1, hT1alpha1, Lung type I cell membrane associated glycoprotein, Lung type I cell membrane associated glycoprotein isoform a, hT1alpha2, PA2.26 antigen, T1 alpha, Lung type I cell membrane associated glycoprotein T1A 2, OTS 8, OTTHuMP00000009640, OTTHuMP00000044504, T1 ALPHA GENE, TIA 2, TIA2

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human PDPN (1-206aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,ICC/IF,FACS
Usage
The antibody has been tested by ELISA, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of Podoplanin in HaLe cells. The cell was stained with APD0833 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Flow cytometry (FACS)

Flow cytometry analysis of Podoplanin in HeLa cells. The cell was stained with APD0833 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.