Human PP2C gamma antibody

Cat No.
APP0902

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
k1G6
UniProt No.
O15355
NCBI Accession No.
NP_002698
Alternative names
Protein phosphatase 1G, PP2CG, PPP2CG, MGC1675, MGC2870, PP2Cgamma, Protein phosphatase 1G, PPM1G, Protein phosphatase 1G, PP2C, PP2C gamma, PPM1G, Protein phosphatase 1C, Protein phosphatase 2C gamma isoform, Protein phosphatase 2 catalytic subunit gamma isoform, Protein phosphatase magnesium dependent 1 gamma.

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human PPM1G (317-546aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF,IHC
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of PPM1G in 293T cells. The cell was stained with APP0902 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The 293T cell lysate (20ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IHC Comment And Pic

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPM1G (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.