NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

J2E1

UniProt No.

P09429

NCBI Accession No.

NP_002119

Alternative names

Sulfoglucuronyl carbohydrate binding protein, SBP-1, HMGB1, HMG3, HMG1, High-mobility group box1, High-mobility group (nonhistone chromosomal) protein 1, DKFZp686A04236, Amphoterin

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human HMGB1 (1-215aa) purified from Baculovirus

Isotype

IgG2b kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, IHC

Usage

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

Magnify

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HMGB1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate
Lane 1.: K562 cell lysate
Lane 1.: MCF7 cell lysate

IHC Comment And Pic

Magnify

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human HMGB1 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Related Publications

Hashizume H, et al. Skin recruitment of monomyeloid precursors involves human herpesvirus-6 reactivation in drug allergy. (Allergy. 2013)
Ito H, et al. HMGB1 facilitates repair of mitochondrial DNA damage and extends the lifespan of mutant ataxin-1 knock-in mice. 9EMBO Mol Med. 2015)