Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT4G6
- UniProt No.
- P60709
- NCBI Accession No.
- NP_001092
- Alternative names
- Actin, cytoplasmic 1, Beta-actin
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 0.2mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human ACTB (1-375aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: Jurkat cell lysate
Lane 3.: PC3 cell lysate
Lane 4.: HepG2 cell lysate
Lane 5.: MCF7 cell lysate
Lane 6.: U87-MG cell lysate
Lane 7.: NIH-3T3 cell lysate
Lane 1.: HeLa cell lysate
Lane 2.: Jurkat cell lysate
Lane 3.: PC3 cell lysate
Lane 4.: HepG2 cell lysate
Lane 5.: MCF7 cell lysate
Lane 6.: U87-MG cell lysate
Lane 7.: NIH-3T3 cell lysate
The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Kidney tissue lysate
Lane 2.: Liver tissue lysate
Lane 3.: Spleen tissue lysate
Lane 4.: Bone marrow tissue lysate
Lane 5.: Stomach tissue lysate
Lane 1.: Kidney tissue lysate
Lane 2.: Liver tissue lysate
Lane 3.: Spleen tissue lysate
Lane 4.: Bone marrow tissue lysate
Lane 5.: Stomach tissue lysate
The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: ACTB antibody 1:1000
Lane 2.: ACTB antibody 1:3000
Lane 3.: ACTB antibody 1:5000
Lane 4.: ACTB antibody 1:10000
Lane 1.: ACTB antibody 1:1000
Lane 2.: ACTB antibody 1:3000
Lane 3.: ACTB antibody 1:5000
Lane 4.: ACTB antibody 1:10000
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.