Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT1C1
- UniProt No.
- P23528
- NCBI Accession No.
- NP_005498
- Alternative names
- 18 kDa phosphoprotein(p18), CFL, CFL1, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, P18
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human Cofilin 1 (1-166aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of Cofilin1 in HeLa cells. The cell was stained with ACO0927(1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: K562 cell lysate
Lane 3.: MCF7 cell lysate
Lane 4.: LnCap cell lysate
Lane 5.: PC3 cell lysate
Lane 1.: HeLa cell lysate
Lane 2.: K562 cell lysate
Lane 3.: MCF7 cell lysate
Lane 4.: LnCap cell lysate
Lane 5.: PC3 cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.