Human Cofilin 1 antibody

Cat No.
ACO0927

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT1C1
UniProt No.
P23528
NCBI Accession No.
NP_005498
Alternative names
CFL1 CFL 18 kDa phosphoprotein(p18), CFL1, CFL, Cofilin 1, CFL1, CFL, 18 kDa phosphoprotein(p18) 18 kDa phosphoprotein, CFL 1, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, P18

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Cofilin 1 (1-166aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of Cofilin1 in HeLa cells. The cell was stained with ACO0927(1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: K562 cell lysate
Lane 3.: MCF7 cell lysate
Lane 4.: LnCap cell lysate
Lane 5.: PC3 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.