Human beta-Tubulin antibody

Cat No.
ATGA0196

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT5B2
UniProt No.
Q9BVA1
NCBI Accession No.
NP_821080
Alternative names
DKFZp566F223, FLJ98847, MGC8685, Tubulin beta-2B chain, bA506K6.1, TUBB2B, Tubulin beta 2b, Tubulin beta-2B

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human beta-Tubulin (1-445aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +4C For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:1000) with Texas Red (Red).
Immunofluorescence of A431 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:500) with Alexa Fluor 488 (Green).
Immunofluorescence of Balb/3T3 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:200) with Alexa Fluor 488 (Green).

Western blot analysis (WB)

The cell lysates(40ug) and tissue lysates(35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin (1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate
Lane 2.: A549 cell lysate
Lane 3.: A431 cell lysate
Lane 4.: HepG2 cell lysate
Lane 5.: Mouse brain tissue lyate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.