Human CtBP1 antibody

Cat No.
ATGA0199

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT4D6
UniProt No.
Q13363
NCBI Accession No.
NP_001319
Alternative names
C-terminal-binding protein 1 isoform 1, BARS, C-terminal-binding protein 1 isoform 1 C terminal binding protein 1, CTBP, CTBP1, MGC104684.

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human CTBP1 (1-440aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500. Recommended starting dilution is 1:500
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of CTBP1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)
ICC/IF analysis of CTBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: NIH3T3 cell lysate
Lane 3.: 293T cell lysate
Lane 4.: AGS cell lysate
Lane 5.: Raji cell lysate
Lane 6.: A549 cell lysate
Lane 7.: Jurkat cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.