Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT1G10
- UniProt No.
- Q8TF65
- NCBI Accession No.
- NP_060125.4
- Alternative names
- PDZ domain containing family member 2, SEMCAP2
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human GIPC2 (1-315aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, FACS
- Usage
- The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Western blot analysis (WB)
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GIPC antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: GIPC1 recombinant protein
Lane 2.: GIPC2 recombinant protein
Lane 1.: GIPC1 recombinant protein
Lane 2.: GIPC2 recombinant protein
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GIPC antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: HepG2 cell lysate
Lane 3.: HeLa cell lysate
Lane 1.: 293T cell lysate
Lane 2.: HepG2 cell lysate
Lane 3.: HeLa cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of GIPC in HeLa cells. The cell was stained with ATGA0223 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.